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Pregnancy following homologous prepubertal ovarian transplantation in the dog


In several canine models of hereditary human disease the homozygote dogs die prior to puberty, or have substantially reduced fertility. To create a clinically healthy animal that can be bred, but can also transmit the gene of interest, a model of homologous ovarian transplantation in prepubertal dogs was developed. Six dog leukocyte antigen (DLA) identical littermates underwent transplantation of ovarian cortical strips (n = 2) or the entire ovary (n = 4). Immunosuppression was maintained with cyclosporine and MMF in the immediate post-operative period and cyclosporine alone thereafter. All 6 dogs entered puberty and normal semiannual estrus cycles as demonstrated by both physical changes and increasing serum progesterone. Four dogs were bred to a proven stud male, and one became pregnant. Three viable fetuses with observable heartbeats were detected on ultrasound examination. Although the dog eventually aborted the litter, this work represents the first pregnancy achieved following a prepubertal ovarian transplant in the dog.



dog leukocyte antigen


luteinizing hormone.


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This work was supported by NIH 5R24HD047142 to Dr. Pullium. The authors thank Dr. Richard Fayrer-Hoskins for helpful comments.

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Correspondence to Jennifer K Pullium.

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The authors declare that they have no competing interests.

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JKP, RM, and GAT conceived, designed, and performed the experiments. JKP interpreted the results and wrote the paper. All authors read and approved the final manuscript.

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Pullium, J.K., Milner, R. & Tuma, G.A. Pregnancy following homologous prepubertal ovarian transplantation in the dog . J Exp Clin Assist Reprod 5, 1 (2008).

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