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Pregnancy following homologous prepubertal ovarian transplantation in the dog

Abstract

In several canine models of hereditary human disease the homozygote dogs die prior to puberty, or have substantially reduced fertility. To create a clinically healthy animal that can be bred, but can also transmit the gene of interest, a model of homologous ovarian transplantation in prepubertal dogs was developed. Six dog leukocyte antigen (DLA) identical littermates underwent transplantation of ovarian cortical strips (n = 2) or the entire ovary (n = 4). Immunosuppression was maintained with cyclosporine and MMF in the immediate post-operative period and cyclosporine alone thereafter. All 6 dogs entered puberty and normal semiannual estrus cycles as demonstrated by both physical changes and increasing serum progesterone. Four dogs were bred to a proven stud male, and one became pregnant. Three viable fetuses with observable heartbeats were detected on ultrasound examination. Although the dog eventually aborted the litter, this work represents the first pregnancy achieved following a prepubertal ovarian transplant in the dog.

Abbreviations

DLA:

dog leukocyte antigen

LH:

luteinizing hormone.

References

  1. Pullium JK, Lin PH, Pinter MJ: Homologous transplantation of prepubertal testes in the dog. Transplantation. 2001, 72 (3): 552-553. 10.1097/00007890-200108150-00040.

    Article  PubMed  CAS  Google Scholar 

  2. Hewitt DA, England GC: Manipulation of canine fertility using in vitro culture techniques. J Reprod Fertil Suppl. 2001, 57: 111-125.

    PubMed  CAS  Google Scholar 

  3. Donnez J, Dolmans MM, Pirard C, Van Langendonckt A, Demylle D, Jadoul P, Squifflet J: Allograft of ovarian cortex between two genetically non-identical sisters: case report. Hum Reprod. 2007, 22 (10): 2653-2659. 10.1093/humrep/dem211.

    Article  PubMed  CAS  Google Scholar 

  4. Silber SJ, Gosden RG: Ovarian transplantation in a series of monozygotic twins discordant for ovarian failure. N Engl J Med. 2007, 356 (13): 1382-1384. 10.1056/NEJMc066574.

    Article  PubMed  CAS  Google Scholar 

  5. Silber SJ, Lenahan KM, Levine DJ, Pineda JA, Gorman KS, Friez MJ, Crawford EC, Gosden RG: Ovarian transplantation between monozygotic twins discordant for premature ovarian failure. N Engl J Med. 2005, 353 (1): 58-63. 10.1056/NEJMoa043157.

    Article  PubMed  CAS  Google Scholar 

  6. Stevens LC: A modification of Robertson's technique of homoiotopic ovarian transplantation in mice. Transplant Bull. 1957, 4 (3): 106-107.

    PubMed  CAS  Google Scholar 

  7. Cunliffe-Beamer TL: Biomethodology and surgical techniques. The Mouse in Biomedical Research. Edited by: Foster HL, Small JD, Fox JG. 1983, New York , Academic Press, 3: 402-

    Google Scholar 

  8. Gosden RG, Baird DT, Wade JC, Webb R: Restoration of fertility to oophorectomized sheep by ovarian autografts stored at -196 degrees C. Hum Reprod. 1994, 9 (4): 597-603.

    Article  CAS  PubMed  Google Scholar 

  9. Baird DT, Campbell B, de Souza C, Telfer E: Long-term ovarian function in sheep after ovariectomy and autotransplantation of cryopreserved cortical strips. Eur J Obstet Gynecol Reprod Biol. 2004, 113 Suppl 1: S55-9. 10.1016/j.ejogrb.2003.11.023.

    Article  PubMed  Google Scholar 

  10. England GCW: Allen's Fertility and Obstetrics in the Dog. 1998, Oxford , Blackwell Science Ltd., 245-Second

    Google Scholar 

  11. England GC, Russo M: Ultrasonographic characteristics of early pregnancy failure in bitches. Theriogenology. 2006, 66 (6-7): 1694-1698. 10.1016/j.theriogenology.2006.01.028.

    Article  PubMed  CAS  Google Scholar 

  12. Davidson AP, Feldman EC: Ovarian and estrous cycle abnormalities in the bitch. Textbook of Veterinary Internal Medicine. Edited by: Ettinger SJ, Feldman EC. 1995, Philadelphia , W. B. Saunders, 2: 1607-1613. 4th

    Google Scholar 

  13. Purswell BJ: Differential diagnosis of canine abortion. Curr Vet Ther Small Anim Pract. 1992, 11: 925-929.

    Google Scholar 

  14. Johnston SD, Root Kustritz MV, Olson PNS: Canine and Feline Theriogenology. 2001, Philadelphia , W. B. Saunders Company

    Google Scholar 

  15. Kemppainen RJ, Thompson FN, Lorenz MD, Munnell JF, Chakraborty PK: Effects of prednisone on thyroid and gonadal endocrine function in dogs. J Endocrinol. 1983, 96 (2): 293-302.

    Article  CAS  PubMed  Google Scholar 

  16. Scott JR, Hendrickson M, Lash S, Shelby J: Pregnancy after tubo-ovarian transplantation. Obstet Gynecol. 1987, 70 (2): 229-234.

    PubMed  CAS  Google Scholar 

  17. Carmona F, Balasch J, Gonzalez-Merlo J: Ovarian function, tubal viability and pregnancy after tubo-ovarian transplantation in the rabbit. Hum Reprod. 1993, 8 (6): 929-931.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by NIH 5R24HD047142 to Dr. Pullium. The authors thank Dr. Richard Fayrer-Hoskins for helpful comments.

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Correspondence to Jennifer K Pullium.

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The authors declare that they have no competing interests.

Authors' contributions

JKP, RM, and GAT conceived, designed, and performed the experiments. JKP interpreted the results and wrote the paper. All authors read and approved the final manuscript.

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Pullium, J.K., Milner, R. & Tuma, G.A. Pregnancy following homologous prepubertal ovarian transplantation in the dog . J Exp Clin Assist Reprod 5, 1 (2008). https://doi.org/10.1186/1743-1050-5-1

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  • DOI: https://doi.org/10.1186/1743-1050-5-1